The DPGP 7MB Project Objectives

A step along the way to the long-term goal of sequencing the euchromatic genomes of 50 independently sampled Drosophila melanogaster strains by high-density-oligonucleotide-microarray-based resequencing was to target a specific region. In this experiment 7 Mbp in each of 50 strains was targeted to validate and improve the platform, to establish a resequencing pipeline, and to develop the capacity and needed analytic tools for a population genetics project of this scale.

We are currently in the process of analyzing the data and preparing a manuscript

Downloads and Supplimentary Material

The sample of 50 D. melanogaster genomes sequenced consists of 40 highly inbred lines derived from a well studied North Carolina population and 10 lines from Malawi (chromosomes were genetically extracted into 27 stocks).

Target Regions

  • Chromosome 2L: 12537331 to 15942868
  • Chromosome X: 1911660 to 5005056

Resequencing Data Release 1.0

The data was generated using RATools an open source model based approach. Sequence in these files had a minimum phred equivalent quality score of 30.